Since it’s possible to correct such a systematic error, we should! If we can measure the PSF, or guess it, we can correct the raw image for it. This is a systematic error, characterized by the Point Spread Function (PSF) of the optical system, which makes the image intensity information non-quantitative. Large features are bright, but small features appear less contrasted and dimmer than they should. The problem, and the solutionĪny optical image forming system, such as a microscope objective lens, has the nasty property of killing more and more contrast of smaller and smaller features, up to the resolution (diffraction) limit, after which there is no contrast (and thus no resolution). Please contact the moderators of this subreddit if you have any questions or concerns.Deconvolution corrects the systematic error of blur (loss of contrast in smaller features) in optical systems such as fluorescence microscopy images. I am a bot, and this action was performed automatically.
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